[Seminar] Non-invasive monitoring of single-cell biophysical properties and bioenergetics during the cell cycle
Time : August 10(Mon) 2020 11:00 ~ 12:00
Place : N7 1floor Central Conference Building(#1601)
Speaker : Joon Ho Kang (KIST)
Non-invasive monitoring of single-cell biophysical properties and bioenergetics during the cell cycle
Joon Ho Kang
Center for BioMicrosystems, Korea Institute of Science and Technology (KIST)
Cells undergo dynamic changes in cell size, shape and mechanics during the cell cycle. However, studying the biophysical dynamics is challenging because both the magnitude and duration of the changes are heterogeneous across the cell population. In this talk, we present new methods to monitor single-cell mechanical properties and bioenergetics during the full cell cycle with temporal resolution of ~1 min. We utilize the suspended microchannel resonator (SMR) which is a fluid-filled cantilever capable of measuring cell buoyant mass by the change of SMR resonant frequency.
First, we quantify mechanical properties via acoustic scattering of waves from a cell inside the SMR. Through simulations, experiment with hydrogels and chemical perturbation of cells, we show that our readout from acoustic scattering measures stiffness. Cells maintained constant stiffness throughout interphase but displayed dynamic changes during mitosis.
Next, we monitor mitochondrial membrane potential of single cells and demonstrate that mitochondrial membrane potential increases during mitosis. By using an electrical circuit model of mitochondria, we quantify the time-dynamics of mitochondrial membrane potential under normal and perturbed conditions to extract mitochondrial ATP synthesis rates in mitosis. We found that mitochondrial ATP synthesis decreases by approximately 50 % during early mitosis and increases back to G2 levels during cytokinesis.
문의: 기계공학과 이정철 교수 (Tel: 3212)